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Typical Plasmid Vector

This post categorized under Vector and posted on July 5th, 2018.

History. The term plasmid was introduced in 1952 by the American molecular biologist Joshua Lederberg to refer to any extrachromosomal hereditary determinant. The terms early usage included any bacterial genetic material that exists extrachromosomally for at least part of its replication cycle but because that description includes bacterial Background. When choosing what plasmid backbone to use you have many elements to consider. Here is a guide to Addgenes empty vector backbones.

In molecular biology subcloning is a technique used to move a particular DNA sequence from a parent vector to a destination vector.. Subcloning is not to be confused with molecular cloning a related technique.Plasmid vectortiCas9-Blast from Dr. Feng Zhangs lab contains the inserts Cas9 and Blasticidin resistance and is published in Nat Methods. 2014 Aug11(8)783-4. doi 10.1038nmeth.3047.NucleoSpin Plasmid EasyPure Plasmid Mini preps. Features. For ultra-fast small scale preparation of up to 35 g of high-purity plasmid DNA LyseControl for visualization of completed alkaline lysis

Plasmid DNA isolation Miniprep kit - high purity high capacity (40 g) fast procedure (18 Minipreps in 25 min) high- and low-copy plasmids made in GermanyFigure 9-A-3. A typical plasmid vector. It contains a polylinker which can recognize several different restriction enzymes an ampicillin-resistance gene (amp r) for selective amplification and a replication origin (ORI) for proliferation in the host cell.

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