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Episomal Vectors

This post categorized under Vector and posted on May 29th, 2018.
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Common Uses of AAV. AAV is commonly used in optogenetics experiments. These viruses are preferred over lentiviruses because they remain primarily episomal while lentiviruses integrate into the genome.Plasmids are the most-commonly used bacterial cloning vectors. These cloning vectors contain a site that allows DNA fragments to be inserted for example a multiple cloning site or polylinker which has several commonly used restriction sites to which DNA fragments may be ligated.REVIEW ARTICLE . A role for adeno-associated viral vectors in gene therapy . Renata dos Santos Coura Nance Beyer Nardi. Laboratrio de Imunogentica Departamento de Gentica Universidade Federal do Rio Grande do Sul Porto Alegre RS Brazil

Adeno-associated virus (AAV) is a small virus that infects humans and some other primate species. AAV is not currently known to cause disease. The virus causes a very mild immune response lending further support to its apparent lack of pathogenicity.Choose from thousands of products that convert the latest innovations into robust easy-to-use research tools. Click here to visit our website.Delivering high titer lentivirus preps Optimized for effective high-titer production of pseudoviral particles SBIs 293TN Producer Cell Line has been transformed with the SV40 large T antigen to promote robust growth and features the neomycin resistance marker for stable propagation. The 293TN packaging cell line is shipped at low passage (P2).

Plasmid pCP20 problem - (reply 1) Creating empty vector - (reply 5) cloning vector and restriction mapping - (reply 2) software to replace Vector NTI and a simple cloning problem - (reply 3)( Adeno-associated virusAAV)(20nm)

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