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In Comparison To Virus Derived Vectors Non Viral Vectors Have Several Advantageousfig

This post categorized under Vector and posted on January 9th, 2019.
Non-Viral Vectors Gene Gun: In Comparison To Virus Derived Vectors Non Viral Vectors Have Several Advantageousfig

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Non-viral methods present certain advantages over viral methods with simple large scale production and low host immunogenicity being just two. Previously low levels of transfection and expression of the gene held non-viral methods at a disadvantage however recent advances in vector technology have yielded molecules and techniques with transfection efficiencies similar to those of viruses .A) Viral plasmids have been engineered such that they can be delivered to a host and generate virus (right) similar to how natural viruses can be delivered to a host and generate virus (left). B) Viral plasmids can be modified for research purposes such that when delivered to a host they give rise to viruses with modified viral genomes.Adeno-graphicociated viral vectors Similarly to adenoviral vectors adeno-graphicociated viral (AAV) vectors can deliver genetic material to dividing and non- dividing cells. It is a small virus that is known to affect humans with a very mild immune response. As a result AAV vectors have beneficial properties for gene therapy that are effective with limited negative effects. However the

Depending on the duration planned for the treatment type and location of target cells and whether they undergo division or are quiescent different vectors may be used involving nonviral methods non-integrating viral vectors or integrating viral vectors (reviewed by El-Aneed 2004 Zubler 2006).


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