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Standard Cloning Vector Puc P

This post categorized under Vector and posted on January 30th, 2020.
PUC18 Vector: Standard Cloning Vector Puc P

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pUC19 is a small high copy cloning vector for replication in E. coli.It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322.The pMB1 of pUC19 differs from the pBR322 origin by a single point mutation and the lack of the rop gene leading to a high copy number. pUC19 has a multiple cloning site within the lacZ alpha-fragment. pUC118 is a small high copy cloning vector for replication in E. coli.It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322.The pMB1 of pUC118 differs from the pBR322 origin by a single point mutation and the lack of the rop gene leading to a high copy number. pUC118 has a multiple cloning site within the lacZ alpha-fragment. Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18.

pUC19 is a commonly used plasmid cloning vector in E.coli. The molecule is a small double-stranded circle 2686 base pairs in vectorgth and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. They are the standard cloning vectors and the ones most commonly used. Most general plasmids may be used to clone DNA insert of up to 15 kb in size. One of the earliest commonly used cloning vectors is the pBR322 plasmid. Other cloning vectors include the pUC series of plasmids and a large number of different cloning plasmid vectors are available. Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning.

pUC19 carries antibiotic resistance gene ampicillin. pUC19 has N-terminal fragment of -galactosidase gene of (LacZ) E.coli. Multiple Cloning Site (MCS) Multicloning site is split in the pUC8 cloning vector Nomenclature- p stands for plasmid - UC stands for University of California where it was constructed 8 stands for order of discovery in the same laboratory Construction- It is derived from pBR322 by just making some slight changes in it. Origin of replication and ampicillin resistant gene remains the same in pUC8 as PGEM3Z plasmid . pGEM3Z is having similarity with a pUC vector because of the presence of the amp R genes and lacZ genes containing a cvectorer of restriction sites. The difference is that pGEM3Z has two additional short pieces of DNA each of which acts as the recognition site for attachment of an RNA polymerase enzyme. Der Vektor codiert fr das N-terminale Fragment (Aminosuren 1-5 und 8-60) der -Galactosidase (lacZ) von E. coli. Die Codons 6-7 wurden durch MCS ersetzt und erlauben so eine Blau-Wei-Selektion. Die beiden pUC-Plasmide unterscheiden sich lediglich in der inversen Orientierung der MCS.
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